TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant feature(s)a | Reference or sourceb |
|---|---|---|
| Strains | ||
| Rhodobacter sphaeroides | ||
| 2.4.1 | WT strain | NBRC |
| Δccr mutant | In-frame deletion mutant of RSP_0960 | This study |
| ΔphaC1 mutant | In-frame deletion mutant of RSP_0382 | This study |
| Escherichia coli | ||
| HST02 | Cloning strain | TaKaRa |
| BL21(DE3) | Protein production strain | Novagen |
| S17-1 | Conjugation strain | NBRC |
| Plasmids | ||
| pUC18 | Cloning vector, Apr | TaKaRa |
| pET22b (+) | Expression vector in E. coli, Apr | Novagen |
| pETRcAceB | Production of C-terminal His6-tagged RcAceB, Apr | This study |
| pK19mobsacB | Mobilizable suicide vector for gene deletion, Kmr | 42 |
| pKDRsccr | pK19mobsacB backbone, deletion of RSP_0960, Kmr | This study |
| pKDRsphaC1 | pK19mobsacB backbone, deletion of RSP_0382, Kmr | This study |
| pMG170 | Shuttle vector stably maintained in R. sphaeroides, Kmr | 43 |
| pMG180 | lac promoter removed from pMG170, Kmr | This study |
| pMGccr | pMG180 backbone, RSP_0960 with 241-bp upstream region, Kmr | This study |
| pMGphaC1 | pMG180 backbone, RSP_0382 with 350-bp upstream region, Kmr | This study |
| pMGP | pMG180 backbone, puc promoter from R. capsulatus, Kmr | This study |
| pMGPaceBA | pMGP backbone, aceBA from R. capsulatus, Kmr | This study |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance.
b
NBRC, National Institute of Technology and Evaluation (NITE) Biological Resource Center.