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(A) Schematic graph of wild-type and mutant putative binding sites of miR-1296-5p targeting the 3′-UTR of CDK6 and EGFR. (B) Relative luciferase activity of SGC-7901 and GSC-803 cells after cotransfection with wild-type or mutant CDK6 and EGFR 3′-UTR reporter genes and miR-1296-5p-mimics. Bar graphs represent the mean ± SEM; n.s. indicates not statistically significant; *P<0.05 and **P<0.01 vs. NC-mimics.
