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. 2019 Jan 10;7(1):e13958. doi: 10.14814/phy2.13958

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© 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Subchronic N‐acetylcysteine (NAC) does not attenuate nicotine‐seeking behavior. (A) A timeline of experimental procedures including nicotine self‐administration, extinction training, reinstatement of nicotine‐seeking, NAC administration, and tissue collection (to be used for either RT‐qPCR, western blots, or dendritic spine morphology). (B) Rats acquired lever pressing to the active lever to receive intravenous infusions of nicotine (0.02 mg/kg/infusion) paired with a light+tone compound stimulus during self‐administration. During extinction training, active lever pressing decreased to inactive lever press rates due to no programmed consequence. (C) No difference was found for the total number of infusions received throughout self‐administration, prior to NAC or vehicle treatment. (D) Administration of NAC during extinction had no significant effect on active lever pressing. (E) In a 2‐h cue‐reinstatement session, animals receiving 0 or 100 mg/kg NAC showed no significant effects of treatment on mean active lever pressing. (F) Upon examination of the time‐course of active lever pressing during the 2‐h reinstatement session, NAC treatment did not significantly reduce cumulative active lever pressing compared to vehicle‐treated animals. *P < 0.0001, significant main effect of session (extinction vs. reinstatement). The bar in (E) indicates a significant main effect of session.