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. 2018 Dec 14;11(1):e9283. doi: 10.15252/emmm.201809283

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© 2018 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Representative double‐immunofluorescence confocal images of coronal sections of the dorsal striatum showing p62 immunolabeling (red) in choline acetyltransferase (ChAT)‐positive (cyano) ChIs of Tor1a ^+/+ and Tor1a ^+/− mice. (A1) Two representative ChIs identified by the white box in the low‐magnification images (scale bar = 20 μm) are shown at higher magnification in (A2) (scale bar = 5 μm). (A3) The graph reporting mean ± SEM values of p62/ChAT signal overlap in ChIs, normalized to Tor1a ^+/+ values of the same experiment, shows a significant increase in p62 in Tor1a ^+/− neurons (Tor1a ^+/+ n = 20 cells, N = 6 mice; Tor1a ^+/− n = 20 cells, N = 6 mice, t‐test ***P = 0.00008).