Figure 2.

LPS-induced inflammatory injury of HaCaT cells was alleviated by Angelica polysaccharide (AP) pre-treatments. (a) HaCaT cells were stimulated with 0–150 μg/mL AP for 48 h, and cell viability was determined by CCK-8 assay. Cells were stimulated with 10 μg/mL LPS or 100 μg/mL AP plus 10 μg/mL LPS. Non-treated cells acted as control. Then, (b) cell viability, (c) percentage of apoptotic cells, (d) expression of proteins associated with apoptosis, and (e) mRNA and (f) protein expressions of inflammatory cytokines were measured by CCK-8 assay, flow cytometry assay, western blot analysis, RT-qPCR, and Western blot analysis, respectively. Data were presented as the mean ± SEM of three independent experiments. CTRL: control.

*P < 0.05; **P < 0.01; ***P < 0.001.