Figure 5.

Effects of Angelica polysaccharide (AP) pre-treatments on HaCaT cell were reversed by SIRT1 knockdown. (a) HaCaT cells were transfected with pEX-2, pEX-SIRT1, sh-NC, or sh-SIRT1 and expression of SIRT1 was measured by western blot analysis. Transfected and untransfected cells were stimulated with 10 μg/mL LPS or 100 μg/mL AP plus 10 μg/mL LPS. Non-treated cells acted as control. Then, (b) cell viability, (c) percentage of apoptotic cells, (d) expression of proteins associated with apoptosis, and (e) mRNA and (f) protein expressions of inflammatory cytokines were measured by CCK-8 assay, flow cytometry assay, western blot analysis, RT-qPCR, and western blot analysis, respectively. Data were presented as the mean ± SEM of three independent experiments. CTRL: control.

*P < 0.05; **P < 0.01; ***P < 0.001.