Table 2.

Detailed results of all constants of the sample dataset consisting of the 2 subsets with 5 binding curves each (SimA and SimB).

Results of Evaluation Method 1 (kobs linearization)
Data	c (reagent) [nM]	kass [1/t*nM]	StErr (kass) [1/t*nM]	kdiss [1/t]	StErr (kdiss) [1/t]	KD [nM]	StErr (KD) [nM]
SimA	25-500	6.75E–05	7.05E–07	7.98E–03	1.46E–04	118.30	2.49
SimB	25-500	6.72E–05	8.60E–07	8.22E–03	1.77E–04	122.35	3.07
Results of Evaluation Method 2 (single–curve analysis)
Data	c (reagent) [nM]	kass [1/t*nM]	StErr (kass) [1/t*nM]	kdiss [1/t]	StErr (kdiss) [1/t]	KD [nM]	StErr (KD) [nM]
SimA	25	8.23E–05	1.36E–05	7.43E–03	1.99E–04	90.32	15.09
SimB	25	1.01E–04	1.51E–05	7.59E–03	2.19E–04	74.90	11.35
SimA	50	7.28E–05	4.79E–06	8.03E–03	1.19E–04	110.30	7.43
SimB	50	7.18E–05	4.83E–06	8.07E–03	1.31E–04	112.47	7.78
SimA	100	6.44E–05	1.85E–06	8.10E–03	8.16E–05	125.80	3.83
SimB	100	6.60E–05	1.83E–06	7.98E–03	7.74E–05	120.87	3.55
SimA	200	6.69E–05	1.01E–06	8.05E–03	5.61E–05	120.45	2.01
SimB	200	6.77E–05	1.04E–06	8.05E–03	5.90E–05	118.92	2.03
SimA	400	6.74E–05	8.28E–07	8.04E–03	4.95E–05	119.25	1.64
SimB	400	6.80E–05	8.34E–07	7.98E–03	4.84E–05	117.41	1.61