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. 2019 Jan 11;20:16. doi: 10.1186/s12882-018-1199-4

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Norfloxacin pretreatment reduces kidney TLR4 protein expression and renal proinflammatory mediators production. (a) Immunohistological staining of TLR4 in the kidney. (b) TLR4/GAPDH protein expression on densitometry. mRNA and protein levels of TNF-α (c, f), IL-6 (d, g) and MCP-1 (e, h) were measured in kidney hemogenates. Data are presented as mean ± SD. One-way ANOVA followed by Bonferroni multiple comparison test was used in all graphs (n = 5 per group). *P < 0.05, **P < 0.01, ***P < 0.001. (Original blots ‘BMC Additional file 1)