Fig. 5.

MiR156 and miR535 families co-targeting SPL gene family in banana. a Sequence alignment of miR156 variants and their targets. The cleavage site detected in the degradome is indicated in blue and yellow letters. b Target plot (t-plot) and alignment of validated targets for mac-miR535a/b and mac-miR535e, respectively. The arrow indicates signatures consistent with miRNA-directed cleavage. Cleavage frequency as determined by gene-specific 5′-rapid amplification of cDNA ends (5’-RACE) at the indicated position is shown in parentheses. Primer sequences used for 5’-RACE are provided in Additional file 1: Table S7. c Achr10T09270 is co-targeted by mac-miR156m and mac-miR535e. The cleavage sites detected in the degradome are highlighted in blue letters