Fig. 4.

Overexpression of miR-30c alleviated high palmitate induced lipotoxicity in vitro. H9c2 cells were transfected with miR-30c mimics/inhibitors (or their control) and then subjected to palmitate (1 mmol/L) stimulation. a Representative Western blots and quantification of PGC-1β, CD36, CPT1B, PDK4, GLUT4 in H9c2 cells with different treatment. β-actin used as an internal control. b Representative images and quantitative analysis of BODIBY 493/503 fluorescent dye staining of neutral lipid level in cells (bar = 25 μm). c ROS generation in cells was examined by flow cytometry with DHE probe treatment. d ATP content of H9c2 cells with different treatment. e Apoptosis of H9c2 cells was measured by flow cytometry with Annexin V-PE and propidium iodide staining. For all panels, data are representative of three independent experiments and expressed as mean ± SEM, *p < 0.05