Fig. 5.

B-Raf reverses the effect of HO-1 knockout on cell cycle. a, b Flow cytometry analysis result (a) and RT-PCR analyses of the indicated cell cycle regulators (b) in A375 cells transfected with B-Raf or treated with 20 μM HO-1 inhibitor (ZnPPIX) for 12 h. c Protein levels of CDK2 and cyclin E in A375 cells transfected with B-Raf or add ZnPPIX. d Comparison of the morphologies of HO-1^−/− cells transfected with B-Raf and control vector. Scale bar: 100 μm. e Flow cytometry analysis of the cell cycle for HO-1^−/− cells transfected with vector and B-Raf. f The mRNA level and protein level of CDK2 and cyclin E were measured by RT-PCR and Western blotting. *P < 0.05; **P < 0.01 by the t-test