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. 2019 Jan 4;9:2899. doi: 10.3389/fimmu.2018.02899

Figure 7.

Figure 7

CCR9 marks populations of Th and Tfh cells with enhanced cytokine expression in autoimmune inflammation. Percentages of cytokine expressing CCR9+ and CCR9− CD4+ T helper (Th) and T follicular helper (Tfh) cells in the GIT and inflamed pancreas of NOD mice. CCR9+ and CCR9− Th cells that contain (A) IL-21, (B) IL-17, (C) IL-22, (D) IFNγ, and (E) TNFα in the spleen. MLN, mesenteric lymph nodes; PP Peyer's Patches; SI IEL, intraepithelial lymphocytes of the small intestine; SI LPL, lamina propria lymphocytes of the small intestine; LI IEL, intraepithelial lymphocytes of the large intestine; LI LPL, lamina propria lymphocytes of NOD mice. Percentages of (F) IL-17 and (G) IL-21 expressing CCR9+ and CCR9− Tfh cells in the mesenteric lymph nodes (MLN) and Peyers patches (PP) of NOD mice. Cytokines were detected by intracellualr immunostaining and FACs analyses. Data is shown as mean ± SD from 3 experiments, female mice 11–12 weeks of age n = 5/group for Tfh cell analyses and n = 7/group for Th cell analyses. Statistical significance was assessed by 2-way ANOVA using Bonferroni's multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001.