Table 1.
Antibodies for Flow Cytometry.
| Molecule | Clone (company) | Label | Dilution |
|---|---|---|---|
| CCR6 | 140706 (BD Biosciences) | Alexa Fluor 647 | 1:200 |
| CCR9 | eBioCW-1.2 (eBioscience) | FITC | 1:150 |
| CD122 | PO3.1 (eBioscience) | PE | 1:100 |
| CD127 | A7R34 (eBioscience) | APC | 1:200 |
| CD3 | 145-2C11 (BD Biosciences) | FITC | 1:200 |
| Pacific Blue | 1:200 | ||
| CD4 | RM4-5 (BD Biosciences) | APC | 1:400 |
| Alexa Fluor 750 | 1:300 | ||
| Pacific Blue | 1:300 | ||
| CD44 | IM7 (eBioscience) | FITC | 1:200 |
| APC | 1:200 | ||
| CD45.2 | A20 (eBioscience) | PeCy7 | 1:300 |
| CD69 | H1.2F3 (BD Biosciences) | FITC | 1:200 |
| CXCR5 | 2G8 (BD Biosciences) | Biotin | 1:100 |
| IFNγ | XMG1.2 (eBioscience) | FITC | 1:200 |
| Pacific Blue | 1:100 | ||
| IL-10 | JES5-16E3 (eBioscience) | APC | 1:200 |
| IL-17A | TC11-18H10 (eBioscience) | FITC | 1:100 |
| IL-21 | Polyclonal (R&D Systems) | Biotin | 1:100 |
| IL-22 | 142928 (R&D Systems) | PE | 1:100 |
| IL-4 | BVD6-24G2 (eBioscience) | FITC | 1:100 |
| IL-9R | 224325 (R&D Systems) | PE | 1:200 |
| PD-1 | J43 (BD Biosciences) | FITC | 1:100 |
| PE | 1:100 | ||
| TNFα | MP6-XT22 (BD Biosciences) | PE | 1:200 |
| α4β7 | DATK32 (BD Biosciences) | PE | 1:200 |