Figure 1.

Time Series Global Transcriptome Analysis of Epithelial Cells and Fibroblasts during Alveologenesis

(A) Experimental scheme of transcriptomic analysis of E18.5, P0.5, P2, P7, P28, and P56 lung epithelial cells and fibroblasts (n = 2 animals except for P56 fibroblasts [n = 1]). FACS, fluorescence-activated cell sorting.

(B) Gating scheme for lung epithelial cells and fibroblasts and purity of cells after cell sorting. Representative plots of P56 mice are shown.

(C) Heatmap of selected AEC2, AEC1, and club cell markers and early lung development-associated genes.

(D) Heatmap of selected fibroblast markers and genes associated with lipids; retinoic acids; and Wnt, Fgf, and Shh signaling.

(E and F) Hierarchical clustering by dendrogram of epithelial cells (E) and fibroblasts (F) based on their transcriptome.

See also Figures S1 and S2, and Tables S7 and S8.