Figure 6.

Olig1 and Sox10 bind synergistically to the mbp promotor in vivo. ChIP assays were performed with 24 hpf, mRNA-injected embryos, fixed with formaldehyde. A, B, Mouse IgG and mouse anti-V5 IgG (A) or mouse anti-Myc IgG (B) were used for immunoprecipitation. PCR was used to detect the mbp promotor region. This fragment was also amplified from the lysate (input), genomic DNA (gDNA), and water control (H₂O). The immunoprecipitated DNA/protein complex was also detected by Western blotting (WB; B).