Fig 5. Colony forming unit (CFU) assay to quantify viable cells in E. faecalis biofilms.
Quantification (Log 10 CFU) of live bacterial cells in 1, 3 and 7 day old E. faecalis FA2-2 and V583 biofilms treated with and without MMP1 under inhibition (a) and disruption (b) conditions. For inhibition experiments, biofilms were grown in presence of MMP1 from day0 to day7. For disruption experiments, biofilms were first grown in BHI media without MMP1 for desired duration, followed by MMP1 treatment. * indicates p-value: *p<0.01, **p<0.01 and ***p<0.001; ns indicates that the effect is not significant. Error bars on the data points represent the standard deviations of 3 technical repeats.