Figure 1.

Nef protected PC12 cells from t‐BHP‐induced cell injury. Cell viability (A) and LDH release (B) were determined by MTT assay and LDH kit (n = 5). ROS generation (C) was determined with a DCFH₂‐DA probe (n = 5). Mitochondrial structure and mitochondrial membrane potential were observed by TEM and high‐content analyser respectively (n = 5) (D). ATP content was determined with a commercial kit (n = 5) (E). Mitochondrial ROS was determined with MitoSOX probes (n = 5) (F). Data are expressed as the mean ± SD and were analysed by ANOVA. *P < 0.05.