Antioxidant effect of Nef in rats subjected to pMCAO. Nef was administered intragastrically 15 min prior to MCAO and 6 h after MCAO. The activity of SOD (A, D), GSH‐Px (B, E) and the concentration of MDA (C, F) were detected by a commercial kit (n = 10). Data are expressed as the mean ± SD and were analysed by ANOVA. *P < 0.05. Serum was labelled with S, and brain tissue was labelled with T.