Figure 4.

Protective effect of Nef on mitochondria in rats subjected to pMCAO. Nef was administered intragastrically 15 min prior to MCAO and 6 h after MCAO. (A) Mitochondrial structure was observed by TEM (n = 5). Mitochondrial vitality (B) was determined with fluorescence dye resazurin (n = 10). Mitochondrial membrane potential (C), activity of Na⁺‐K⁺‐ATPase (E) and Ca²⁺‐Mg²⁺‐ATPase (F) were determined with commercial kits (n = 10). Mitochondrial respiration (D) was determined by Oxygraph‐2k (n = 10). Data are expressed as the mean ± SD and were analysed by ANOVA. *P < 0.05.