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. 2018 Dec 18;176(3):400–415. doi: 10.1111/bph.14537

Figure 6.

Figure 6

Nef activated the Nrf2 pathway in PC12 cells and rats subjected to pMCAO. The translocation of Nrf2 to the nucleus in PC12 cells was determined by immunofluorescence (n = 5) (A). The expressions of n‐Nrf2, Keap1 and HO‐1 in PC12 cells were determined by Western blotting (n = 5) (B, C). Cell viability with or without ZnPP and Nef was determined by MTT assay (n = 5) (D). The expressions of n‐Nrf2, Keap1, HO‐1 and NQO1 in rats were determined by Western blotting (n = 5) (E, F). Data are expressed as the mean ± SD and were analysed by ANOVA. *P < 0.05. n‐Nrf2, nucleus‐Nrf2; n.s., no significance; ZnPP, zinc protoporphyrin.