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Nef induced autophagy in PC12 cells subjected to t‐BHP and rats subjected to pMCAO. Autophagic vacuoles were determined by MDC staining (A) and observed by TEM (B) (n = 5). The expressions of p62 and LC3 with or without t‐BHP in PC12 cells were determined by Western blotting (n = 5) (C, D). The expressions of p62 and LC3 in rats were determined by Western blotting (n = 5) (E). Data are expressed as the mean ± SD and were analysed by ANOVA. *P < 0.05. n.s., no significance.
