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Nef protected PC12 cells from t‐BHP injury through the activation of p62–Keap1–Nrf2. The expressions of n‐Nrf2, p62, Keap1 and LC3 were detected by Western blotting (n = 5) (A, C). The translocation of Nrf2 to the nucleus was determined by immunofluorescence (n = 5) (B). Connections among p62, Keap1 and Nrf2 were detected by immunoprecipitation (n = 5) (D). Data are expressed as the mean ± SD and were analysed by ANOVA. *P < 0.05. n‐Nrf2, nucleus‐Nrf2; n.s., no significance.
