Figure 3.

Concentration dependency of the rate of flucloxacillin hydroxylation as catalysed by 0.5 mg·mL⁻¹ pooled HLM (A), 100 nM CYP3A4 Supersomes (B), 100 nM CYP3A7 Supersomes (C) and 100 nM CYP2C9 Supersomes (D). Each condition was incubated in duplicate, and each data point represents as mean whereas error bars represent the range of duplicate measurements. Enzymes kinetic parameters obtained by non‐linear regression according to the Michaelis–Menten equation were (A) HLM: K _M 284 ± 38 μM and V_max 30 ± 2 pmol·min⁻¹·mg⁻¹ protein; (B) CYP3A4 Supersomes: K _M 124 ± 15 μM and V_max 197 ± 9 pmol·min⁻¹·nmol⁻¹ P450, (C) CYP3A7 Supersomes: K _M of 65 ± 8 μM and V_max 193 ± 7 pmol·min⁻¹·nmol⁻¹ P450 and (D) CYP2C9 Supersomes: K _M of 508 ± 82 μM and V_max 67 ± 7 pmol·min⁻¹·nmol⁻¹ P450.