REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-CD56 PE (RRID: AB_10598372)	eBioscience	Cat#12–0567
Anti-NKp44 AlexaFluor647 (RRID: AB_647153)	BDPharmingen	Cat#558564
Anti-CD103 BV605 (RRID: AB_2564283)	Biolegend	Cat#350218
Anti-CD94 PE-Cy7 (RRID: AB_2632752)	Biolegend	Cat#305515
Anti-H3K27ac (RRID: AB_2118291)	Abcam	Ab4729
Anti-CD3 FITC (RRID: AB_314060)	Biolegend	Cat#300406
Anti-CCR6 BV421 (RRID: AB_2561356)	Biolegend	Cat#353408
Anti-CD3 PerCP-Cy5.5 (RRID: AB_1518743)	eBioscience	Cat#45–0036
Anti-CD19 PerCP-Cy5.5 (RRID: AB_2043821)	eBioscience	Cat#45–0199
Anti-CD94 FITC (RRID: AB_314534)	Biolegend	Cat#305504
Anti-NKp44 PE (RRID: AB_647239)	BDPharmingen	Cat#558563
CD56 Microbeads	Miltenyi Biotec	Cat#130–050-401
Anti-NKp44 PE-Cy7	Biolegend	Cat#325116
Anti-CD127 BV421	Biolegend	Cat#351309
Anti-CD117 BV510	Biolegend	Cat#313219
Anti-CCR7 PE	Biolegend	Cat#353204
Anti-S100A4 PE	Biolegend	Cat#370003
Anti-CXCR2 PE	Biolegend	Cat#320705
Anti-AHR unconjugated	Santa Cruz Biotechnology	Cat#sc-133088
Anti-mouse IgG2b PE	SouthernBiotech	Cat#109209
Anti-TIGIT BV605	Biolegend	Cat#372711
Anti-CD27 PE	eBioscience	Cat#12–0279-42
Anti-CD6 FITC	BDPharmingen	Cat#559491
Anti-CD57 FITC	eBioscience	Cat#11–0577-41
Anti-TCF1 PE	Biolegend	Cat#655207
Anti-Ksp37 PE	Biolegend	Cat#346606
Anti-CD57 biotin	Biolegend	Cat#346606
Anti-IL18R biotin	Biolegend	Cat#313806
Anti-CX3CR1 PE	Biolegend	Cat#341604
Anti-CD62L BV605	Biolegend	Cat#304833
Anti-Gata3 PE	eBioscience	Cat#12–9966-41
Anti-CD56 APC	eBioscience	Cat#17–056-42
Anti-RUNX2	Cell Signaling Technology	Cat#12556S
Anti-Rabbit PE	SouthernBiotech	Cat#4050–09
Streptavidin BV421	BDPharmingen	Cat#563259
DX5 microbeads	Miltenyi	Cat#130–052-501
Anti- mouse NK1.1 BV421	Biolegend	Cat#108731
Anti-mouse CD3 APC	eBioscience	Cat#17–0031-83
Anti-mouse CD19 APC	BDPharmingen	Cat#550992
Anti-mouse CD11b PE	eBioscience	Cat#12–0112-85
Anti-mouse CD27 FITC	eBioscience	Cat#11–0271-81
Cytof Panel
NKp44–167Er	Ebioscience	Cat#16–3369-85
CD45–089y	Fluidigm	Cat#3089003B
CD196–141Pr	Fluidigm	Cat#3141003A
CD19–142Nd	Fluidigm	Cat#3142001B
CD117–143Nd	Fluidigm	Cat#3143001B
CD4–145Nd	Fluidigm	Cat#3145001B
CD8a-146ND	Fluidigm	Cat#3146001B
CD25–149Sm	Fluidigm	Cat#3149010B
FceR-150Nd	Fluidigm	Cat#315001B
CD138–150Nd	Fluidigm	Cat#3150012B
CD103–151Eu	Fluidigm	Cat#3151011B
TCRgd-152S	Fluidigm	Cat#3152008B
TIGIT-153Eu	Fluidigm	Cat#3153019B
CD3–154Sm	Fluidigm	Cat#3154003B
CD85j-156Gd	Fluidigm	Cat#3156020B
CD194–158Gd	Fluidigm	Cat#3158006A
CD161–159Tb	Fluidigm	Cat#3159004B
CD39–160Gd	Fluidigm	Cat#3160004B
CD27–162Dy	Fluidigm	Cat#3162009B
CD45RO-165Ho	Fluidigm	Cat#3165011B
CD34–166Er	Fluidigm	Cat#3166012B
CD127–168Er	Fluidigm	Cat#3168017B
CD159–169Tm	Fluidigm	Cat#3169013B
CD45RA-170Er	Fluidigm	Cat#3170010B
CD226–171Yb	Fluidigm	Cat#3171013B
CD354–172Yb	Fluidigm	Cat#3172022B
CD94–174Yb	Fluidigm	Cat#3174015B
CD56–176Yb	Fluidigm	Cat#3176003B
CD16–299Bi	Fluidigm	Cat#3209002B
CD7–147Sm	Fluidigm	Cat#3147009B
PD1–155Gd	Fluidigm	Cat#3155007B
CD26–161Dy	Fluidigm	Cat#3161003B
CD57–163Dy	Fluidigm	Cat#3163003B
CD158–173Yb	Fluidigm	Cat#3173002B
ICOS-148Nd	Fluidigm	Cat#3148019B
Cisplatin	Enzo life Sciences	Cat#NC0503617
CyPBS	Rockland	Cat#MB-008
BSA	Sigma	Cat#A3059
Sodium Azide	Sigma	Cat#71289
EDTA	Hoefer	Cat#GR123–100
PFA 16%	Electtron Microscopy Sciences	Cat#201192A
Intercalator-Ir125	Fluidigm	Cat#201192A
Biological Samples
Adult Blood	Missisipi Valley Regional Blood Center, MO
Human tonsils, as surgical waste	Children Hospital St Louis, MO
Chemicals, Peptides, and Recombinant Proteins
7α,25-dihydroxycholesterol	Avanti Polar	Cat#700080
TRizol	Invitrogen	15596026
T4 DNA Polymerase	New England Biolabs	M0203L
Klenow DNA Polymerase	New England Biolabs	M0210L
T4 PNK	New England Biolabs	M0201L
Klenow (3’−5’ Exo-)	New England Biolabs	M0212L
Quick DNA Ligase	New England Biolabs	M2200L
Phusion HF Master Mix	New England Biolabs	M0531L
Recombinant Human IL-12p80	Peprotech	Cat#20012p80H
Recombinant Human IL-18	Peprotech	Cat#200–18
Critical Commercial Assays
Nextera DNA Library Preparation Kit	Illumina	FC-121–1030
Nextera Index Kit	Illumina	FC-121–1011
NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina	NEB	E6240L
EZ nuclei isolation kit	Sigma	NUC101–1KT
Low Bind DNA Tubes	Ambion	AM12450
MaxTract High Density Tubes	Qiagen	129046
Agencourt AMPure XP	Beckman Coulter	A63880
RNeasy Plus Micro kit	Qiagen	Cat#74034
Human Th1/Th2/Th17 Cytokine Kit BD Cytometric Bead Array	BD Biosciences	Cat#560484
Deposited Data
Raw and Analyzed Data	This paper	GSE112813
ATAC- and H3K27ac-seq of ieILC1 and ILC3	Koues O.I. and Collins P.L. et al 2016	GSE77299
Microarray of Zeb2 Knockout CD8 cells	Dominguez et al. 2015	GSE72408
Mouse CD11b+/− and CD27+/− NK RNA-seq	Immgen consortium	GSE109125
Mouse CD11b+/− and CD27+/− NK ATAC-seq	Immgen consortium	GSE100738
Mouse NK BLIMP1 ChIP-seq	Mackay L.K. et al, 2016.	GSE79339
Human GM12878 TBX21, and K562 eGFP-ZEB2 or TCF7 ChIP-seq	ENCODE Consortium	https://www.encodeproject.org/
Mouse NK TBX21 ChIP-seq	Shish, HY et al, 2017	GSE77695
Mouse CD8 PRDM1 and MAF knockout expression data	Chihara et al., 2018	GSE113968
Macaque expression data	Hong et al 2013	Private Communication
Software and Algorithms
Bowtie2	Langmead and Salzberg, 2012	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
Samtools	Li et al., 2009	http://samtools.sourceforge.net/
Novoalign	Hercus et al, 2012	www.novocraft.com
R	R open license	https://www.r-project.org/
pvclust	Suzuki et al, 2006	https://cran.r-project.org
ggplot2	Wickham H., 2016	http://ggplot2.tidyverse.org/
OrthoRetriever	Kasprzyk A., 2011	lighthouse.ucsf.edu/orthoretriever/
Picard	MIT open license	https://broadinstitute.github.io/picard/
MACs2	Zhang Y et al, 2008	https://github.com/taoliu/MACS
ChIPSeeker	Yu G. Et all, 2015	http://bioconductor.org
HOMER	Heinz S. et al, 2010	http://homer.ucsd.edu/homer/
CrossMap	Zhao, H. et al, 2013	crossmap.sourceforge.net/
BEDTools	Quinlan A.R. and Hall I.M, 2010	bedtools.readthedocs.io
UCSC Genome Browser	Karolchik et al, 2003	https://genome.ucsc.edu/
Monocle	Qiu et al, 2017	https://bioconductor.org/
Gene Ontology	Ashburner et al, 2000	http://www.geneontology.org/