Figure 1:

Frontal lobe white matter depletion of oligodendrocyte lineage cells following early life chemotherapy

A) Photomicrographs of Olig2⁺ (brown) cells in frontal cortex white matter of a child exposed to chemotherapy at 3 years old and non-chemotherapy exposed aged-matched control

B) Chemotherapy exposure selectively depletes Olig2⁺ cells in frontal lobe white matter (p=0.0211; n=4) but not grey matter (p=0.0913; n=4)

C) Olig2⁺ cells throughout early life and young adulthood following chemotherapy treatment compared to age-matched controls

D) Confocal photomicrographs of PDGFRα⁺/NG2⁺ cells in the frontal lobe subcortical white matter of a 10 year old male who received no chemotherapy (left) and a 3 year old male treated with high-dose methotrexate (MTX) chemotherapy in the same anatomical region (right)

Data shown as mean±SEM, n.s. = p>0.05, * p<0.05 by paired t-test; n=4/group. Scale bars=20 μm. See also Table S1