Figure 4:

Microenvironmental perturbation drives differentiation of oligodendrocyte precursor cells

A) Schematic illustration of juvenile chemotherapy paradigm and syngeneic transplantation of GFP⁺/PDGFRα⁺ cells

B) Confocal photomicrograph of transplanted GFP/PDGFRα cells in the corpus callosum 10 days-post transplantation (GFP=green; PDGFRα=red; Olig1=white; Scale bar=20 μm)

C) Syngeneic transplantation of GFP/PDGFRα cells into previously MTX- (n=3 mice) or PBS-exposed (n=4 mice) corpus callosi. Percent of cells GFP/PDGFRα (p=0.01167), GFP/PDGFRα/Olig1^nuclear (p=0.259), and GFP/Olig1^perinuclear oligodendrocytes (p=0.00295) in MTX- and PBS-exposed mice 10 days-post transplantation. Data shown as mean±SEM; unpaired two-tailed Student’s t-test. See also Figure S4