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. 2019 Jan 11;51(1):6. doi: 10.1038/s12276-018-0199-6

Fig. 3. Activation of mitophagy induces apoptosis in HSCs.

Fig. 3

a Mitochondrial DNA (mtDNA) measured by PicoGreen staining in LX2 cells; scale bar, 10 μm. b Representative western blots of TOM20 with GAPDH serving as the internal reference. Bar graph represents the mean ± SEM. *P < 0.05 vs. the indicated groups. c TUNEL staining of LX2 cells from the indicated groups; scale bar, 25 μm. Bar graph represents the mean ± SEM. *P < 0.05 vs. the indicated groups. d Annexin V-FITC/PI double-staining and flow cytometry analysis of LX2 cells. Bar graph represents the mean ± SEM. **P < 0.01 vs. the indicated groups. e Representative western blots of cleaved caspase3, cleaved caspase9, collagen I and α-SMA. Bar graph represents the mean ± SEM of three different experiments. *P < 0.05 and **P < 0.01 vs. the indicated groups