Figure 5.

Expression of CEA by MC38 Parental Cells and CEA-Expressing MC38-CEA Cells and Tumor Challenge Study with MC38 Parental Cells following rAAV-CEA + pGM-CSF Adjuvant Applications

(A) CEA expression on MC38 parental cells was compared to that expressed by MC38-CEA cells to confirm lack of CEA expression by parental cells. (B) Cohorts of naive mice, mice that received pGM-CSF adjuvant injections only, or mice that were vaccinated with rAAV-CEA plus pGM-CSF adjuvant injections were tumor challenged with MC38 parental cells (4 × 10⁵) and assessed for tumor development, starting 1 week post-tumor challenge (n ≥ 3).