Fig. 7.

MI day 7 fibroblasts showed an anti-angiogenic myofibroblast phenotype profile. a Volcano plot and major upregulated processes. b Thbs1 expression was significantly increased; n = 3 per group. c LV infarct region from MI day 7 stained for cardiac fibroblast marker PDGFRα (green), Thbs1 (red), and DAPI (blue). Thbs1 was significantly increased within regions staining positive for PDGFRα; n = 4 day 0, n = 5 MI day 7. d Immunoblot analysis of Thbs1 in cardiac fibroblast secretome. MI significantly increased cardiac fibroblast secretion of Thbs1 at MI day 7; n = 3 per group; densitometry normalized to total membrane stain. *p < 0.05 versus day 0; ^$p < 0.05 versus MI day 3