Fig. 1.

Identification of FUNDC2 as a mitochondrial protein. a Protein sequence alignment of FUNDC2 from different species using ClustalW. b Mitochondrial localization of FUNDC2. Mouse embryonic fibroblasts (MEFs) expressing HA-tagged FUNDC2 were stained with an HA antibody and MitoTracker. Scale bar, 10 μm. c Immunoblot analysis of HeLa cytosolic (Cyto) or mitochondrial (Mito) fractions. d FUNDC2 is localized on the outer mitochondrial membrane. HeLa mitochondria expressing FUNDC2 with C-terminal myc and N-HA were immunoblotted. e Schematic diagram of FUNDC2 topology. TM transmembrane domain, OMM outer mitochondrial membrane