Figure 2.

A low protein diet alters the hepatic transcriptome and shows distinct changes in biological pathways. (A–C) RNA-Seq was performed on the livers for mice fed a Control diet or a Low AA for four months. (A) A heatmap indicating the relative expression of genes involved in the most significantly enriched biological KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways based on genes differentially expressed in the livers of Control and Low AA fed mice (q < 0.05, FDR). Genes in more than one significantly enriched KEGG pathway are listed only once, and assigned to the most significantly affected pathway. (B) Pathway enrichment analysis was performed using g:Profiler (g:GOSt)⁷³, and the p-values of KEGG pathways significantly up- and downregulated by Low AA diet feeding were determined. Colors are matched to that of pathways in (A). (C) Heatmap representing the relative expression of liver genes known to be altered by FXR-FGF15 bile acid signaling.