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. 2019 Jan 11;9:67. doi: 10.1038/s41598-018-37177-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A low protein diet alters body composition similarly in vehicle and antibiotic-treated mice. (A) Schematic representation of the experimental plan; mice were pretreated with antibiotics or vehicle for three weeks, and then randomized to either a Control or Low AA diet. (B) Fecal DNA content was determined following 3 weeks of antibiotic treatment (n = 8/group; *p < 0.05, t-test). (C) Weight of the mice in each group was tracked following randomization to each diet. (D–F) Weight and body composition were determined immediately prior to diet start and after 6 weeks on the indicated diets, and the change in (D) weight, (E) fat mass, and (F) lean mass was determined (n = 12/group; statistics for the overall effects of diet, antibiotic (ABX) treatment, and the interaction represent the p-value from a two-way ANOVA; *p < 0.05 from a Sidak’s post-test examining the effect of parameters identified as significant in the two-way ANOVA). Error bars represent SEM.