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. 2019 Jan 11;10:137. doi: 10.1038/s41467-018-08150-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Combination of splicing modulator E7107 with BCLxL inhibitors induces synergistic cytotoxicity. a 8 × 8 dose matrix combination study for E7107 and ABT263 in MCL1-dependent NCIH23 cells stably transduced with vector control (Vector) or BCL2L1 shRNA. Synergy scores were calculated by the Chalice software (Horizon Discovery). One representative of three independent experiments is shown. b Western blot analysis of MCL1, BCLxL, cleaved PARP1 (cPARP), and GAPDH (loading control) in MCL1-independent A549 cells treated with E7107 or BCLxL-specific inhibitor A1155463. c Growth curves measured by CellTiter-Glo (CTG) in A549 cells treated with single agent (black curves: left, E7107; right, A1155463) or in combination (red curves: left, E7107+40 nM A1155463; right, A1155463+40 nM E7107). Data represent means ± SD of biological triplicates. d 8 × 8 dose matrix combination study for E7107 and BCLxL/BCL2 inhibitors (A1155463, ABT263, and ABT199) in A549 cells stably transduced with vector control (Vector) or BCL2L1 shRNA. Synergy scores were calculated by Chalice. One representative of three independent experiments is shown