Table 3.

Detected methylated motifs

Genome ID	Detected methylated motif	Modification type	Motif in REBASE	Number of methylated sites	Number of motif sequences	Methylation ratio (%)	Mean modification QV	Mean subread coverage
BS1	GANTC	m6A	Yes	1813	2070	87.6	58.0	35.2
	TTAA	m6A	Yes	1264	1522	83.0	55.5	34.1
	GCWGC	m4C	Yes	3026	15,948	19.0	38.4	40.6
BS3	GANTC	m6A	Yes	3724	4014	92.8	66.1	41.3
	TTAA	m6A	Yes	3036	3338	91.0	62.4	40.4
	GCWGC	m4C	Yes	13,821	54,026	25.6	39.5	46.4
BS8	AGGNNNNNRTTT	m6A	No	80	276	29.0	39.6	65.8
BS10	ACGAG	m6A	No	1986	7185	27.6	45.0	171.4
BS12	GMAGCTKC	m4C	No	169	220	76.8	50.9	83.5
	HCAGCTKC	m4C	No	124	293	42.3	46.8	79.0
	BGMAGCTGD	m4C	No	78	185	42.2	46.3	76.3
BS14	GANTC	m6A	Yes	2856	2880	99.2	190.6	166.9
BS15	GAANNNNTTC	m6A	Yes	1309	1472	88.9	55.6	30.9
	AGCNNNNNNCAT	m6A	No	642	726	88.4	56.0	29.4
	ATGNNNNNNGCT	m6A	No	619	726	85.3	52.0	29.8
	AGCNNNNNNGTG	m6A	No	311	349	89.1	56.9	30.4
	CACNNNNNNGCT	m6A	No	293	349	84.0	53.3	30.9
	CAANNNNNNNNCTTG	m6A	No	205	256	80.1	49.4	29.1
	CAAGNNNNNNNDTTG	m6A	No	164	214	76.6	48.7	28.7
	TTAGNNNNNCCT	m6A	No	87	99	87.9	51.3	29.8
	AGGNNNNNCTAA	m6A	No	77	99	77.8	49.4	29.7
	GYTANNNNNNNTTRG	m6A	No	76	89	85.4	56.0	31.3
	CYAANNNNNNNTAVCH	m6A	No	59	127	46.5	53.5	32.6
BD1	GCWGC	m4C	Yes	72,730	77,932	93.3	140.2	297.3
	GANTC	m6A	Yes	6754	6844	98.7	346.3	281.7
	TTAA	m6A	Yes	5475	5564	98.4	325.3	270.9
BD2	TANGGAB	m6A	No	1276	1367	93.3	64.4	48.5
BD3	GATC	m6A	Yes	9446	9618	98.2	122.1	93.7
	AGCT	m4C	Yes	5974	6224	96.0	84.0	92.1

R = A/G, M = A/C, W = A/T, S = C/G, Y = C/T,  K = G/T, H = A/C/T, B = C/G/T, D = A/G/T, V = A/C/G,  N = A/C/G/T

Underlined bold face indicates methylation sites