Table 4.

Detected MTases, REases, and specificity subunit genes

Genome ID	CDS ID	Gene type	Top-hit protein in REBASE	Identity (%)	Recognition motif of the closest-match MTase	Modification type	RM type	RM system	TRD divergence	Motif detected	MTase name	Confirmed recognition motif
BS3	EMGBS3_04270	M	M.SstE37II	58.9	GANTC	m6A	II	No	No	Yes
	EMGBS3_09240	M	M.Sth20745I	71.4	TTAA	m6A	II	No	No	Yes
	EMGBS3_12600	M	M1.BceSIII	22.9	ACGGC	m4C	II	No	Yes	No	M.AbaBS3I	GCWGC
BS6	EMGBS6_08960	M	M.SinI	57.0	GGWCC	m5C	II	No	No	No
BS8	EMGBS8_10720	R	DvuI	36.3	?	–	I	–	–	–
	EMGBS8_10740	S	S.PveNS15I	32.4	?	–	I	–	Yes	–
	EMGBS8_10750	M	M.RbaNRL2II	55.6	ACGANNNNNNGRTC	m6A	I	Yes	–	No
BS10	EMGBS10_10070	RM	CjeFIII	23.7	GCAAGG	m6A	II	Yes	Yes	No	M.ObaBS10I	ACGAG
BS14	EMGBS14_10020	M	M.Bsp460I	56.7	GANTC	m6A	II	No	No	Yes
BS15	EMGBS15_02830	M	M.Bli37I	56.6	GAYNNNNNRTC	m6A	I	Yes	–	No
	EMGBS15_02840	M	M.EcoNIH1III	59.2	GATGNNNNNNTAC	m6A	I	Yes	–	No
	EMGBS15_02870	S	S.PveNS15I	47.2	?	–	I	–	Yes	–
	EMGBS15_02930	R	DvuI	38.4	?	–	I	–	–	–
	EMGBS15_03820	M	M.EcoGI	25.8	Nonspecific	m6A	II	Yes	Yes	No	M.FspBS16I	GAANNNNTTC
	EMGBS15_03830	R	XmnI	34.0	GAANNNNTTC	–	II	–	–	–
	EMGBS15_04560	R	GmeII	33.8	TCCAGG	–	III	–	–	–
	EMGBS15_04600	M	M.FpsJII	53.4	CGCAG	m6A	III	Yes	No	No
	EMGBS15_05670	M	M.FnuDI	59.8	GGCCa	m4C	II	Yes	No	No
	EMGBS15_05690	R	BhaII	45.6	GGCC	–	II	–	–	–
	EMGBS15_12460	M	M.Mva1261III	37.1	CTANNNNNNRTTC	m6A	I	No	No	No
BD1	EMGBD1_08400	M	M.Sth20745I	71.0	TTAA	m6A	II	No	No	Yes
	EMGBD1_09320	M	M1.BceSIII	22.9	ACGGC	m4C	II	No	Yes	No	M.AbaBS3I	GCWGC
	EMGBD1_19510	M	M.SstE37II	58.9	GANTC	m6A	II	No	No	Yes
BD2	EMGBD2_08760	M	M.HgiDII	55.0	GTCGACa	m5C	II	Yes	No	No
	EMGBD2_08790	RM	AquIV	28.5	GRGGAAG	m6A	II	Yes	Yes	No	M.NbaBD2I	TAHGGAB
	EMGBD2_08800	R	LpnPI	56.3	CCDG	–	II	–	–	–
BD3	EMGBD3_00670	M	M.Mma5219II	45.9	AGCT	m4C	II	No	No	Yes
	EMGBD3_01960	M	M.AvaVI	50.3	GATC	m6A	II	No	No	Yes

Underlined bold face indicates methylation sites

M: methyltransferase, R: restriction endonuclease, S: specificity subunit

^aModified base undetermined