Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 11;10:177. doi: 10.1038/s41467-018-08179-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Characterization of visual responses of different layers in V1 of awake mice. a Three-dimensional rendering of a sequence of 450 lateral three-photon images acquired with 2-µm increment. Green color represents GCaMP6s signal, and magenta color represents label-free THG signal generated in the blood vessels in the visual cortex and myelin fibers in the white matter. Scale bar, 100 µm. b Selection of lateral images from layer 2/3, 4, 5, and 6. Scale bar, 50 µm. Field of view in all lateral images is 250 μm. c Average calcium responses (ΔF/F) for representative cells in each layer over 10 trials in response to oriented gratings moving in specific directions (arrows above each trace) and their orientation tuning curves in polar plots. Discontinuities in (ΔF/F) traces are due to the randomization of each stimulus direction in each trial. (ΔF/F) scale bars correspond to 100% and time scale bar corresponds to 10 s as shown in the bottom right panel