Fig. 6.

Effects of insulin in the perfused mouse pancreas. a Glucagon secretion measured before and after addition of insulin using the perfused mouse pancreas preparation. For these experiments, wild-type mice were used. In each experiment, glucagon secretion was normalised to that at 4 mM glucose prior to the addition of insulin (100 nM in three experiments and 10 μM in two experiments: responses have been pooled for display; female mice were used). b As in a but the insulin receptor antagonist S961 was used instead of insulin (n = 7 female mice). c As in a but experiments were performed in hyperglycaemic/diabetic Fh1βKO mice and using insulin at a concentration of 100 nM (n = 8 mice of both sexes). In a-c, the black bars represent mean glucagon secretion measured during the 6 min preceding the addition of insulin or S961. d As in (c) but CYN154806 was included as indicated (n = 4 diabetic Fh1βKO mice of both sexes). Glucagon secretion has been normalised to that in the presence of CYN154806 (measured at steady-state, 4–8 min after the addition; t = 4–8 min). The red and bars represent glucagon secretion in the presence of insulin alone and following the addition of CYN154806, respectively. Statistical significances were analysed by Student’s t-test. *p < 0.05. Data are presented as dot plots of individual experiments and/or mean values ± S.E.M. of all experiments with the experimental series