Fig. 5.

HMR’s TAD is required for the activation of thermoresponsive genes. a qRT-PCR analyses of the steady-state levels of warm-temperature-induced PIF4 direct target genes, YUC8, IAA29, and IAA19, in 4-d-old Col-0, pif4-2, hmr-5, and hmr-22 seedlings grown under 27 °C in Rc, LD (R-LD), and SD (R-SD) conditions with 10 μmol m⁻² s⁻¹ R light. Samples were taken at 96 h after stratification for Rc, 104 h (or ZT 8) for LD, and 94 h (or ZT 22) for SD. Error bars represent SD of three replicates. Different letters denote statistically significant differences in transcript levels (ANOVA, Tukey’s HSD, P < 0.01). b qRT-PCR analyses of the transcript levels of YUC8, IAA29, and IAA19 in response to elevated temperatures in Col-0, pif4-2, and hmr-22. Four-day-old Col-0, pif4-2, and hmr-22 seedlings grown at 10 μmol m⁻² s⁻¹ Rc at 21 °C were transferred to 27 °C under the same light condition for up to 24 h, and samples were collected at the indicated time points. Error bars represent SD of three replicates. For all the qRT-PCR analyses, the transcript levels were calculated relative to those of PP2A. The source data of the qRT-PCR data in a and b are provided in the Source Data file