Fig. 9.

VE-cadherin/Y658 phosphorylation is essential in the ability to form VM: a VE-cadherin and Y658 VE-cadherin expression in MUM 2B and MUM 2B VE-cadherin K.O, b, c In vitro angiogenesis assay with Matrigel in Scb, siVE-Cad, VE-Cad K.O, VE-Cad K.O+WT VE-Cad construct, and VE-Cad K.O+Y658F VE-Cad construct in MUM 2B showed that VE-cadherin is essential for the formation of VM, images were acquired using an Olympus CKX41 microscope (bars 50 μm) and the formation of tube-like structures was then quantified by Wimasis program. Each treatment was performed in triplicate, and the experiment was independently repeated at least three times, d) VM formation as measured by the capacity to form loops quantified by the Wimasis software. **p<0.01, ***p<0.001