Fig. 5.

Selective blockade of the SC–vlPul pathway by using two viral vectors. a The retrograde gene transfer viral vector (HiRet-TRE-eGFP.eTeNT) was injected into the vlPul and the anterograde viral vector (AAV1-CMV-rtTAV16) was injected into the SC. Then double infection occurs only on the neurons whose cell bodies were in the SC and whose axons projected to the vlPul. The synaptic transmission from SC neuron to vlPul is selectively blocked under the administration of Dox. See Supplementary Figure 5 and Methods for detail. b Representative locations of the HiRet injections. Red dot indicates the injection site and volume (inset). See Supplementary Figure 6 for all the injections. c Above: Nissl-stained histological section corresponding to b. Below: magnified area showing the most dorsal injection track. Black rectangle indicates the magnified area. Scale bar: 2 mm