Fig. 4.
a Lungs from 2- and 18-month-old FVB/N mice were excised, cut into small pieces (2–3 mm3) and assessed for β-gal positivity (blue colour). b 2- and 18-month-old FVB/N mice were treated once a week i.p. with MitoTam (2 μg of MitoTam/1 g of mouse) dissolved in 4% EtOH in corn oil or solvent control with the excipient for 4 weeks, lungs were excised, cut into small pieces (2–3 mm3) and assessed for β-gal activity (blue colour). A length of 80 μm thin sections were then statistically analysed for β-gal positivity (c). Expression of p16Ink4a, p21waf1 and PAI genes in lungs (d) and kidney (e) were estimated by qRT-PCR. The asterisk represents p < 0.05