Fig. 7.

Administration of interleukin-1 receptor antagonist attenuates motor dysfunctions and dopaminergic neuronal loss in MPTP-treated mice. a The severity of hindlimb clasping in MPTP-treated mice with or without IL-1Ra administration (n = 10). b The latent time to hold the bar in the catalepsy test in MPTP-treated mice with or without IL-1Ra administration (n = 10). c Quantification of relative TH-positive cells per DAPI in the confocal images as shown in (d) (n = 5). e Quantification of relative Iba1-positive cells in the confocal images as shown in f based on DAPI staining (n = 4 or 5). d, f Representative immunofluorescence image of the fixed brain sections containing the SN of MPTP-treated mice with or without IL-1Ra administration after staining with anti-TH antibody (green, d) or anti-Iba1 antibody (red, f). DAPI represents the nuclear signal (blue). Scale bars, 200 μm. Data were expressed as the mean ± SEM. Asterisks indicate significant differences (*P < 0.05, **P < 0.01)