FIGURE 5:

Ectopic expression of bgcn rescues Nipped-A RNAi early defect. (A, A′) Germline-driven bgcn^EP, (B, B′) depletion of Nipped-A, and (C, C′) rescue germaria stained with Vasa (blue) and 1B1 (red). Germline-driven bgcn^EPand rescue germaria show fusome formation (yellow dashed line). Germaria depleted of Nipped-A accumulate only spectrosome-containing cells (white arrow). 1B1 channel is shown in A′, B′, and C′. (D) Quantitation of the number of single cells in germline-driven bgcn^EP, depletion of Nipped-A, and rescue germaria (2.78 ± 1.38 in bgcn^EP; 5.72 ± 2.37 in rescue compared with 16.52 ± 5.02 in Nipped-A RNAi; n = 50 for all ****P < 0.0001). (E) Schematic showing Tip60 complex regulates differentiation in the precystoblast (GSC daughter). In G2 phase, Pgc is expressed prior to differentiation, we postulate that Tip60 complex modulates bgcn mRNA levels to promote differentiation. Bgcn and Bam proteins then complex to stimulate differentiation in the GSC daughter. Statistical analysis performed with Student’s t test. Scale bar for all images is 20 μm.