Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 26;29(24):2946–2958. doi: 10.1091/mbc.E18-05-0305

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Saito-Reis et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

PMC Copyright notice

FIGURE 4: — CD82KO does not impact CXCR4 expression or activity. Representative histograms of (A) surface and (B) total (permeablized) CXCR4 expression of bone marrow HSPCs from WT and CD82KO mice. Flow cytometry analysis measured the mean fluorescence intensity (MFI) of CXCR4 on the LSK population. Phosphoflow cytometry analysis of (C) basal and (D) tonic (1 h serum starvation) conditions to assess the mean fluorescence intensity (MFI) of pAKT, pERK, and total ERK signaling of the LSK population. n = 3 mice per strain. (E) Phosphoflow cytometry analysis of SDF-1 treatment at various time points post 1 h of serum starvation to assess pAKT, pERK, and total ERK signaling of the LSK population. Quantification ratio calculated by dividing tonic signaling by SDF-1 treatment conditions. n = 3 mice per strain.