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. 2019 Jan 1;15(1):114–126. doi: 10.7150/ijbs.28679

Figure 4.

Figure 4

EGFRvIII's nuclear translocation promoted phosphorylation of STAT3. A: Immunoblotting results of p-STAT3 in U251 and U251EGFRvIII with or without serum-free starvation culture for 24h. Lower histogram was the relative densities of p-STAT3. B: U251EGFRvIII cells were treated with or without 24h serum-free starvation culture, or 24h serum-free starvation culture after adding brefeldin A (BFA) or DMSO for 30min, and then immunoblotting analysis were performed to detect the protein levels of p-STAT3. DMSO is the control of BFA. Lower histogram was the relative densities of p-STAT3. C: Immunoblotting analysis of non-nuclear and nuclear p-STAT3 in C6, C6EGFRvIII and C6EGFRvIII-NLS-del after treating with or without 24h serum-free starvation culture. Lower histogram was the relative densities of nuclear p-STAT3. D: Immunoblotting analysis of p-STAT3 in U251, U251EGFRvIII and U251EGFRvIII-NLS-del after treating with or without 24h serum-free starvation culture. Lower histogram was the relative densities of p-STAT3. E: Immunoblotting analysis of p-STAT3 in C6, C6EGFRvIII and C6EGFRvIII-NLS-del after treating with or without 24h serum-free starvation culture. Lower histogram was the relative densities of p-STAT3.