Figure 4.

Antiviral function of ELAVL4 as a miR-375 host target. (A) The target sequence of the ELAVL4 gene was predicted using the miRanda algorithm. (B) The expression of ELAVL4 was detected by western blotting. (C) DF-1 cells were transfected with pCAGGS-HA-ELAVL4 and vector plasmids (Vec) for 24 h, and infected with F48E9 (0.1 MOI) or rLa Sota-GFP (0.1 MOI). The viral titers of F48E9 and rLa Sota-GFP at various infection points were detected using the TCID₅₀ method. (D) Flow cytometry was performed to analyze changes in the cell cycle after ELAVL4 overexpression and vector (Vec) transfection. (E) A scratch assay was used to verify cell growth indirectly after ELAVL4 overexpression and vector (Vec) transfection. The wound closure percentage was calculated as the relative wound area using Image J. Results are presented as means ± SEM (n = 3). Statistical analyses were performed in GraphPad Prism using unpaired 2-tailed t-tests: *P < 0.05, **P < 0.01, ns. indicates no significant difference.