Figure 8.

Pre-treatment of DF-1 cells with miR-375 has different effects on NDV proliferation. Supernatants containing miR-375, miR-NC, sponge-375, or sponge-NC were used to culture the DF-1 cells at 24 h before incubation with rLa Sota-GFP or F48E9. After another 24 h post-NDV infection, the viral titers were evaluated. Observations (A) and quantitative analyses (B) of fluorescent cells infected with rLa Sota-GFP, and observations (C) and statistical analyses (D) for the plaque assay for F48E9 infection are shown. Results are presented as means ± SEM (n = 3). Statistical analyses were performed in GraphPad Prism using unpaired 2-tailed t-tests: *P < 0.05, ns. indicates no significant difference.