Table 1.
The primers used in this study
| Primers | Sequences (5'-3') | Size (bp) | TM (℃) |
|---|---|---|---|
| pCAGGS-HA-ELAVL4-F | CCATCGATATGGAGTGGAATGGCCTGAAGATG | 1159 | 53 |
| pCAGGS-HA-ELAVL4-R | CGGCTAGCTCAAGCGTAGTCTGGGACGTCGTATGGGTAGGATTTGTGGGTTTTATTGG | ||
| pCAGGS-Flag-LM-F | GGAATTCATGGACTCATCTAGGACAAT | 1135 | 50 |
| pCAGGS-Flag-LM-R | GAAGATCTTTACTTATCGTCGTCATCCTTGTAATCTTTCTTAAAAGGATTGT | ||
| Luc-FM-F | CCGCTCGAGATGGACTCATCTAGGACAATTG | 567 | 52 |
| Luc-FM-R | TTGCGGCCGCTTCGGCACCACAGTCAAAGAAAC | ||
| Luc-LM-F | CCGCTCGAGATGGACTCATCTAGGACAATTG | 567 | 52 |
| Luc-LM-R | TTGCGGCCGCTTCGGTACCACAGTCAAGGAGAC | ||
| M-F | CCGATCGTCCTACAAGACACAG | 223 | 60 |
| M-R | GGACGCTTCCTAGGCAGAGCAT | ||
| NDV-F | ATGGGCYCCAGAYCTTCTAC | 535 | 55 |
| NDV-R | CTGCCACTGCTAGTTGTGATAATCC | ||
| ELAVL4-F | GGTATCGTATGCCCGTCCAA | 214 | 60 |
| ELAVL4-R | GGCTTCTTCCGCCTCTATTC | ||
| ELAVL4-shRNA1F | GATCCGGAATGGCCTGAAGATGATAA TTCAAGAGATTATCATCTTCAGGCCATTCCTTTTTTg | 63 | 37 |
| ELAVL4-shRNA1R | AATTCAAAAAAGGAATGGCCTGAAGATGATAATTCAAGAGATTATCATCTTCAGGCCATTCCg | ||
| ELAVL4-shRNA2F | GATCCGGAGTCTCTTTGGGAGCATTG TTCAAGAGACAATGCTCCCAAAGAGACTCCTTTTTTg | 63 | 37 |
| ELAVL4-shRNA2R | AATTCAAAAAAGGAGTCTCTTTGGGAGCATTG TTCAAGAGACAATGCTCCCAAAGAGACTCCg | ||
| shRNA-NC-F | GATCCGACCCGGCGACGGGCTAGTCTTCAAGAGAGACTAGCCCGTCGCCGGGTCTTTTTTg | 63 | 37 |
| shRNA-NC-R | AATTCAAAAAAGACCCGGCGACGGGCTAGTC TTCAAGAGAGACTAGCCCGTCGCCGGGTCg | ||
| miR-375-qF | TTTGTTCGTTCGGCTCGCGTTA | 60 |