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. 2019 Jan 1;15(1):58–68. doi: 10.7150/ijbs.25783

Table 1.

Sequences of primers employed for RT-PCR. RT-PCR primers used with their name, orientation (S, sense; AS, antisense), sequence, annealing temperature (Tm), and length of amplicon (bp).

Name S/AS Sequence Tm (℃) bp
RANKL Forward TGG AAG GCT CAT GGT TGG AT 59 75
Reverse CAT TGA TGG TGA GGT GTG CA
OPG Forward TGG AAG GCT CAT GGT TGG AT 59 75
Reverse CAT TGA TGG TGA GGT GTG CA
CTSK Forward CTTCCAATACGTGCAGCAGA 59 155
Reverse TCTTCAGGGCTTTCTCGTTC
TRAP Forward CACTCCCACCCTGAGATTTGT 60 145
Reverse CCCCAGAGACATGATGAAGTCA
IL-1α Forward GTGTTGCTGAAGGAGTTGCCAGAA 60 156
Reverse GTGCACCCGACTTTGTTCTTTGGT
IL-1β Forward AAGAGCTTCAGGCAGGCAGTATCA 60 190
Reverse TAATGGGAACGTCACACACCAGCA
IL-6 Forward GGACCAAGACCATCCAAT 60 127
Reverse ACCACAGTGAGGAATGTC
IL-8 Forward AACTTCTCCACAACCCTCTG 57 277
Reverse TTGGCAGCCTTCCTGATTTC
MMP-3 Forward CAAAACATATTTCTTTGTAGAGGACAA 57 91
Reverse TTCAGCTATTTGCTTGGGAAA
MMP-13 Forward ACTTTGTTGCCAATTCCAGG 57 131
Reverse TTTGAGAACACGGGGAAGAC
GAPDH Forward AGA AGG TGG TGA AGC AGG CAT C 60 111
Reverse CGA AGG TGG AAG AGT GGG AGT TG