Figure 3.

Calpain-2 promotes the progression of NSCLC via the activation of EGFR-pAKT signaling pathway. (A) The proliferative ability was assessed with CCK-8 assay at 24, 48 and 72 hours after transfection. (B) The proliferative ability was assessed with colony-forming assay and analyzed statistically after 2-week culture. (C) The migration ability was assessed with Transwell assay. (D) The apoptosis of cells was detected with flow cytometry after staining of Annexin V and PI. (E) The proliferative ability was assessed with tumor xenograft assay and analyzed statistically 4 weeks after implantation. (F) Western blot was performed to analyze the expression difference of the related protein after knockdown of Calpain-2. β-actin was used as an internal reference. (ns: no significance, *p <0.05, **p <0.01, ***p < 0.001).